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皇冠8868会员登录|新的基因编辑技术诞生


本文摘要:Scientists in the US have developed a powerful new genome editing system that could offer significantly more precision and efficiency than the current CRISPR standard.美国科学家已产品研发出有强悍的新的基因组编辑系统软件,显著提高现阶段CRISPR规范的精密度和高效率。

Scientists in the US have developed a powerful new genome editing system that could offer significantly more precision and efficiency than the current CRISPR standard.美国科学家已产品研发出有强悍的新的基因组编辑系统软件,显著提高现阶段CRISPR规范的精密度和高效率。While CRISPR-Cas9 is a revolutionary medical technology that lay the modern foundations for editing genetic code – including variants associated with disease – there have long been concerns over its potential for imprecision.尽管CRISPR-Cas9是颠覆性的医疗技术,为编辑还包含与病因学的基因变异以内的遗传密码奠下了当代基本,但依然不会有对其潜在性的不精确性的焦虑。

Specifically, many fear that CRISPR-Cas9 editing carries the potential to introduce errors in the form of uncontrolled insertions and deletions in genetic code, called indels.从总体上便是很多人畏惧CRISPR-Cas9编辑有可能在称之为数据库索引的遗传密码中也不受操控的放进和清除信息进而导致不正确。Researchers say the new system, dubbed prime editing by its inventors at the Broad Institute of MIT and Harvard University, could change the game thanks to a new protein that enables high-precision edits of genetic targets.科学研究工作人员讲到被麻省理工大学和美国哈佛大学的布罗德研究室的发明人称之为“先导编辑”的这新系统能变化遗传基因,有一种新式蛋白使科学研究工作人员能够对总体目标遗传基因进行高精密编辑。A major aspiration in the molecular life sciences is the ability to precisely make any change to the genome in any location, says genome biologist David Liu from the the Broad Institute.布罗德研究室的基因组科学家David Liu讲到:“分子结构生物科学的一个关键总体目标是能精确变化一切方向的基因组。

”Were not aware of another editing technology in mammalian cells that offers this level of versatility and precision with so few byproducts.“我们不告知有别的对哺乳类动物体细胞进行编辑的技术性能像这一技术性一样主要用途广泛、高精度,并且不良反应那么较少。”The basis of the new prime editing method is an enzyme called reverse transcriptase. The CRISPR system also uses an enzyme – Cas9 – to cut DNA strands, so that alternative genetic code can be inserted.这一新的先导编辑方式的基本是称之为逆转录酶的酶,CRISPR系统软件也运用这类Cas9酶切成DNA链,放进取代遗传密码。

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Now, thanks to reverse transcriptase, which is used in conjunction with Cas9 in prime editing, genomic editing has been upgraded again.如今好在拥有逆转录酶,基因组编辑再一次升級了。逆转录酶与Cas9一起被作为先导编辑。In prime editing, a guide RNA called pegRNA guides a modified form of the Cas9 enzyme to snip only a single strand of DNA (preventing the double-strand breaks that can induce unintended disruptions).先导编辑中起名叫pegRNA的推动RNA提醒修改过的Cas9酶只绑一条DNA链(避免双解链导致车祸事故终断)。

After this, the reverse transcriptase enzyme directly copies edited genetic information contained in the pegRNA to the targeted genomic site.随后逆转录酶必需将pegRNA中编辑过的遗传基因信息载入基因组的总体目标方向。


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